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ANNUAL CONFERENCE :: Abstract Library
Abstract Library
2003 Conference Abstract
| Type of Submission |
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Submission Type: |
Poster Presentation |
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Subject Category: |
Infection and Immunity |
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| Session Information |
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Presentation Date: |
May 27, 2003 |
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Abstract ID: |
D25 |
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Session: |
Poster 2 |
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Time: |
15:00 |
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| Presenting Author |
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J.P. BURTON , Canadian Research and Development Centre for Probiotics, The Lawson Health Research Institute, London, Ontario, Canada
blis4@blis.co.nz |
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| Other Authors |
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E. DEVILLARD, Canadian Research and Development Centre for Probiotics, The Lawson Health Research Institute, London, Ontario, Canada
P.A. CADIEUX, Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada
G. REID, Canadian Research and Development Centre for Probiotics, The Lawson Health Research Institute, London, Ontario, Canada
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| Title |
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Atopobium: A Common Inhabitant Amongst the Vaginal Bacterial Microbiota of Post Menopausal Women with High Grade Nugent Scores |
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| Abstract Text |
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In previous studies, bacterial vaginosis has been shown to be a typically polymicrobial infection. Additionally, using PCR-DGGE analysis of bacterial 16S rRNA gene amplicons from vaginal swabs of post menopausal women, we have shown that the microbial consortia often varies greatly between subjects with Nugent scores indicative of bacterial vaginosis. The aim of this study was to characterize the vaginal microbiota of one post menopausal subject with polymicrobial bacterial vaginosis in order to obtain a greater understanding of the composition of microbial populations present during infection.
A subject with bacterial vaginosis as defined by the Nugent criteria was analyzed via PCR-DGGE and determined to possess a polymicrobial bacterial vaginal microbiota. A clone library of 16S rRNA gene sequences from PCR amplicons of bacterial DNA was generated and 62 clones sequenced and identified using database homology searches. In addition, the incidence of the Atopobium genus was determined by specific PCR and amplicon sequencing of DNA isolated from vaginal swabs of non menopausal, post menopausal and post menopausal subjects on hormone replacement therapy.
Amongst the sequenced DNA library clones, sequences matching 13 different bacterial species were detected, comprising 9 genera. Interestingly, those of Atopobium vaginae and related bacteria were the most commonly detected, in addition to other bacteria previously undetected in the vagina, such as Megasphaera sp. PCR screening using Atopium-specific primers found a strong association between the presence of this organism and Nugent scores indicative of bacterial vaginosis amongst the post menopausal subjects. Additionally, the detection of Gardnerella vaginalis strongly correlated with the presence of Atopobium amongst all groups.
In conclusion, 16S rRNA clone libraries appear to be a valuable tool for the identification of bacteria from clinical samples, especially those difficult to culture or low in number. Additionally, Atopobium and other members of the Coriobacteriaciae family may be important in the progression of adverse vaginal health.
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