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ANNUAL CONFERENCE :: Abstract Library
Abstract Library
2003 Conference Abstract
| Type of Submission |
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Submission Type: |
Poster Presentation |
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Subject Category: |
Infection and Immunity |
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| Session Information |
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Presentation Date: |
May 27, 2003 |
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Abstract ID: |
D24 |
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Session: |
Poster 2 |
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Time: |
15:00 |
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| Presenting Author |
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E. DEVILLARD, Canadian Research and Development Centre for Probiotics, The Lawson Health Research Institute, London, Ontario, Canada
edevilla@lri.sjhc.london.on.ca |
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| Other Authors |
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J.P. BURTON, Canadian Research and Development Centre for Probiotics, The Lawson Health Research Institute, London, Ontario, Canada
P.A. CADIEUX, Department of Microbiology and Immunology, University of Western Ontario, London, Ontario
G. REID, Canadian Research and Development Centre for Probiotics, The Lawson Health Research Institute, London, Ontario, Canada
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| Title |
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Identification of pathogenic organisms in bacterial vaginosis, by using PCR-Denaturing Gradient Gel Electrophoresis |
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| Abstract Text |
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Bacterial vaginosis is one of the most frequent gynecological diagnoses encountered by primary care physicians. Its diagnosis is based upon the patient's symptoms, physical examination and the findings of microscopic examination of wetmounts. However, recurrences are common, and there is a need to identify the microorganisms involved in order to prescribe the most appropriate treatment. To illustrate the diversity of the vaginal microflora in bacterial vaginosis, we present findings from a postmenopausal woman with a recurrent vaginal infection. Repeated antimicrobial therapy had not resolved her condition. Vaginal swabs were sampled every week for six weeks and analysed for their microbial composition. Samples were first analysed by microscopic observations. An infectious state was confirmed by very high Nugent scores (index of vaginal bacterial infection) of 9 or 10 (10 being the maximum value of the index). DNA extraction from the swabs followed by PCR-Denaturing Gradient Gel Electrophoresis (DGGE) with universal eubacterial primers showed that the vaginal flora was mostly composed of bacteria with potential pathogenicity. While the microbial population showed overall differences among the six samples, the bacterial composition was identical between the last three. From all samples, two recurrent bacterial species were identified: Klebsiella oxytoca and Morganella morganii. In addition, other uropathogenic species were identified namely Escherichia coli, Kluyvera ascorbata, Serratia fonticola and Citrobacter freundii. No lactobacilii species were detected using universal primers, and only a few strains were found in the samples studied using PCR-DGGE primers specific for lactobacilli. This low level of lactobacilli confirmed the infectious state, as the overall numbers of lactobacilli tend to decrease during bacterial vaginosis, compared to higher levels within an asymptomatic vaginal flora. In summary, PCR-DGGE is useful to identify pathogens responsible for recurrent bacterial vaginosis. Some of these pathogens are also commonly responsible for urinary tract infections. The potential to restore a lactobacilli microflora using probiotics could not only lead to a reduced risk of urinary tract infections, as previously shown, but also resolution of bacterial vaginosis and reduced risk of its recurrence. |
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