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ANNUAL CONFERENCE :: Abstract Library
Abstract Library
2003 Conference Abstract
| Type of Submission |
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Submission Type: |
Poster Presentation |
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Subject Category: |
Environmental Microbiology |
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| Session Information |
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Presentation Date: |
May 27, 2003 |
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Abstract ID: |
B31 |
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Session: |
Poster 2 |
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Time: |
15:00 |
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| Presenting Author |
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S. DUTIL, Centre de recherche, Hôpital Laval, Institut universitaire de cardiologie et de pneumologie de l'Université Laval, Québec, Canada. Département de biochimie et microbiologie, Université Laval, Québec, Canada.
steve.dutil@crhl.ulaval.ca |
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| Other Authors |
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M.P. CORREIA, Département de stomatologie, Faculté de médecine dentaire, Université de Montréal, Montréal, Canada.
C. LAFLAMME, Centre de recherche, Hôpital Laval, Institut universitaire de cardiologie et de pneumologie de l'Université Laval, Québec, Canada.
A. MÉRIAUX, Centre de recherche, Hôpital Laval, Institut universitaire de cardiologie et de pneumologie de l'Université Laval, Québec, Canada.
A. LEDUC, Département de stomatologie, Faculté de médecine dentaire, Université de Montréal, Montréal, Canada.
J. BARBEAU, Département de stomatologie, Faculté de médecine dentaire, Université de Montréal, Montréal, Canada.
C. DUCHAINE, Centre de recherche, Hôpital Laval, Institut universitaire de cardiologie et de pneumologie de l'Université Laval, Québec, Canada. Département de biochimie et microbiologie, Université Laval, Québec, Canada.
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| Title |
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Detection of Legionella sp. by fluorescent in situ hybridization (FISH) in dental units waterlines |
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| Abstract Text |
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Background : Due to low residual chlorine concentration and to water stagnation, dental unit waterlines are exposed to the formation of a biofilm. It has been proposed that this biofilm contributes to the proliferation and the release of pathogens like Legionella pneumophila in the water. Some of those bacteria are fastidious and may not be recovered by culture. Non-culture related methods such as fluorescent in situ hybridization (FISH) may increase their detection. Methods : Twenty three water samples from 15 dental units was analyzed. Three techniques of qualitative detection for Legionella sp. were used : (1) fluorescent in situ hybridization with LEG705 probe, specific to Legionella sp. genus (direct FISH), (2) FISH with a previously R2A enrichment (R2A/FISH) and (3) traditional culture approach on BCYE (buffered charcoal yeat extract) with glycine, vancomycine and polymixine B (GPV). Results : 91% of water samples (21/23) analysed showed a contamination by Legionella sp.. When direct FISH and R2A/FISH were performed, two- and three-fold higher sensitivity were reached, respectively, when compared to the culture approach. Although FISH allows a better recovery than culture, this method may not allow a 100% recovery of the Legionella positive units. Conclusions : Conventional culture may be a source of false-negative results since non-culturable pathogens present in dental unit waterlines may not be recoverable. The detection of Legionella sp. by FISH in dental units water is possible and is more rapid and sensitive than the traditional culture approach. |
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