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ANNUAL CONFERENCE :: Abstract Library
Abstract Library
2003 Conference Abstract
| Type of Submission |
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Submission Type: |
Poster Presentation |
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Subject Category: |
Environmental Microbiology |
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| Session Information |
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Presentation Date: |
May 27, 2003 |
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Abstract ID: |
B28 |
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Session: |
Poster 2 |
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Time: |
15:00 |
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| Presenting Author |
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N. FORTIN, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Ave., Montreal, QC. H4P 2R2.
Nathalie.Fortin@nrc.ca |
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| Other Authors |
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D. OUELLETTE, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Ave., Montreal, QC. H4P 2R2
C. MASSON, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Ave., Montreal, QC. H4P 2R2
L.G. WHYTE, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Ave., Montreal, QC. H4P 2R2
K. LEE, Bedford Institute of Oceanography, Fisheries and Oceans Canada, Darmouth, Nova Scotia.
C.W. GREER, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Ave., Montreal, QC. H4P 2R2
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| Title |
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Microbial Population Structure and Degradation Potential in a Marine Wetland Experimentally Exposed to Crude Oil |
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| Abstract Text |
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The contribution of the indigenous microbial community to the bioremediation of crude oil was evaluated following a controlled oil spill in a Nova Scotia salt marsh. Activity assessment and molecular analyses were used to monitor the microbial population response and recovery in the sediment and rhizosphere, under natural attenuation and nutrient-enhanced conditions. On site analysis of gas flux (nitrous oxide) was used to monitor the denitrification rates in the sediment under various conditions. Total microbial community DNA was extracted and analyzed by the polymerase chain reaction (PCR) for the presence of genes involved in the degradation of aromatic (ndoB)and aliphatic (alkB) hydrocarbons. The dynamics and phylogenetic composition of the community was also examined by denaturing gradient gel electrophoresis (DGGE). Results indicated that the microbial community responded positively to the application of oil and nutrients. The microbial population showed increases in denitrification activity after the application of fertilizers. Molecular analyses demonstrated that the community had the potential to degrade both aromatic and aliphatic hydrocarbons immediately after the oil spill. Populations of bacteria possessing the ndoB gene were not as prevalent 16 weeks after the spill as compared to aliphatic hydrocarbon degrading bacteria. DGGE analyses revealed that the microbial population diversity was similar among treatments, but interesting differences in the community structure were observed, with time, in the natural attenuation treatment and in the nutrient-enhanced plots that were routinely tilled. Although natural attenuation may have been an effective treatment in this case, it may be necessary under other circumstances to provide nutrient and oxygen amendments to stimulate the growth and activity of hydrocarbon-degrading bacteria. |
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