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ANNUAL CONFERENCE  ::  Abstract Library

Abstract Library


2003 Conference Abstract


Type of Submission
Submission Type: Poster Presentation
Subject Category: Environmental Microbiology


Session Information
Presentation Date: May 27, 2003
Abstract ID: B23
Session: Poster 2
Time: 15:00


Presenting Author
K. DROUIN, Biotechnology Research Institute, National Research Council, 6100 Royalmount Ave., Montreal, QC. H4P 2R2
Karine.Drouin@nrc.ca


Other Authors
S. DELISLE, Biotechnology Research Institute, National Research Council, 6100 Royalmount Ave., Montreal, QC. H4P 2R2
S. MOORE, Biotechnology Research Institute, National Research Council, 6100 Royalmount Ave., Montreal, QC. H4P 2R2
S. LABELLE, Biotechnology Research Institute, National Research Council, 6100 Royalmount Ave., Montreal, QC. H4P 2R2
A. MIHOC, Biotechnology Research Institute, National Research Council, 6100 Royalmount Ave., Montreal, QC. H4P 2R2
C.W. GREER, Biotechnology Research Institute, National Research Council, 6100 Royalmount Ave., Montreal, QC. H4P 2R2


Title
Feasibility and pilot study for assessing in situ biostimulation of BTEX and TPH degradation in contaminated groundwater


Abstract Text
The property of CFB Chatham was acquired in 1940 to form part of the training program for the Canadian Armed Forces during World War II. Building 66 located in the study area was used for vehicle maintenance and fuel servicing. Potential sources of contamination in this area were former underground storage tanks (UST’s) and their associated pump island, solvents and a maintenance pit. Previous characterization studies revealed contaminated soil and groundwater impacted with gasoline (BTEX) and total petroleum hydrocarbons (TPH). This site is also located in the wellhead protection area of a potable water production supply. Evaluation of the potential for natural attenuation was performed in 1999 by analyzing groundwater geochemical indicators of biodegradation. Based on known biological processes the calculated BTEX assimilative capacity of the shallow aquifer on the site was not high enough to completely degrade the dissolved BTEX phase. Laboratory work was therefore performed to determine whether hydrocarbon degrading microorganisms were present in the contaminated groundwater by plate count and colony hybridization with an alkB gene probe, indicative of alkane degrading microbial populations. The potential capacity to degrade hydrocarbons under different treatment conditions was also determined by using microcosm experiments conducted under aerobic conditions with, and without, mineral nutrients and two supplementary carbon sources. Nutrients and molasses were the most promising treatments for the remediation of the BTEX and TPH plume. An optimization phase was performed to determine the concentration of molasses that would permit good mineralization without the risk of well clogging. Continued hydrogeological characterization work and a pilot study were performed in the field in 2001 and 2002 to assess the feasibility of the in situ biostimulation of BTEX and TPH degradation in the contaminant plume. The results showed that during the summer and fall 2002, BTEX and TPH concentrations had decreased significantly in several monitoring wells located in the pilot test area.


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