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ANNUAL CONFERENCE :: Abstract Library
Abstract Library
2003 Conference Abstract
| Type of Submission |
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Submission Type: |
Poster Presentation |
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Subject Category: |
Applied Microbiology |
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| Session Information |
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Presentation Date: |
May 26, 2003 |
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Abstract ID: |
A18 |
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Session: |
Poster 1 |
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Time: |
14:00 |
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| Presenting Author |
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| Other Authors |
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H. IWAKI, BRI, NRC
H. BERGERON, BRI, NRC
P.C.K. LAU, BRI, NRC
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| Title |
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A ReactIR study of cyclododecanone monooxygenase-catalyzed reaction for lauryl lactone production |
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| Abstract Text |
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ReactIR4000 is a state-of-the-art tool that allows real-time, in-situ analysis of a biotransformation or bioconversion and it can provide a characteristic fingerprint of specific molecular information that reflects changes in a reaction as a function of time. We have applied this technique to monitor the progress of a Baeyer-Villiger monooxygenase (BVMO) reaction. BVMOs are flavoproteins and they are nature’s alternative to the abiotic Baeyer-Villiger organic reaction of ketones into esters or of cyclic ketones into lactones by peracids. BVMO applications include the production of chiral building blocks especially those of lactones. As part of a biocatalyst "toolkit" development program, we have cloned a cyclododecanone monooxygenase-encoding gene (cdnB) from a soil microorganism, Pseudomonas putida strain HI-70. The production of lauryl lactone from cyclododecanone using an overexpression clone of cdnB gene (pCD100B) in Escherichia coli strain BL21 is evaluated in this study. This preliminary study includes the development of a two-phase partition system that addresses low reactant solubility and product inhibition. |
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