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ANNUAL CONFERENCE :: Abstract Library
Abstract Library
2003 Conference Abstract
| Type of Submission |
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Submission Type: |
Poster Presentation |
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Subject Category: |
Infection and Immunity |
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| Session Information |
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Presentation Date: |
May 27, 2003 |
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Abstract ID: |
D7 |
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Session: |
Poster 2 |
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Time: |
15:00 |
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| Presenting Author |
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| Other Authors |
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J. WILLIAMS, NRC Institute for Marine Biosciences
S. SPERKER, NRC Institute for Marine Biosciences
A. DACANAY, NRC Institute for Marine Biosciences
M. REITH, NRC Institute for Marine Biosciences
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| Title |
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Type IV pili are virulence factors for Aeromonas salmonicida infection of Atlantic Salmon |
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| Abstract Text |
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Aeromonas salmonicida is the causative agent of the fish disease furunculosis, it can infect a wide variety of fish species, but is particularly important as a pathogen of Atlantic salmon. We (the NRC IMB) are sequencing the genome of A. salmonicida. Although no pili are seen in electron micrographs, our genome sequencing program has identified four pili operons in this genome (three type IV and one type I). One of the type IV pili operons is homologous to the Vibrio cholerae MshA pili operon; however, it has a large internal deletion (9 of 17 genes) and thus may not be expressed. The second operon is homologous to the Caulobacter crescentus Cpa and Actinobacillus acetomycetemcomitans Flp pili operons. In these organisms the Flp pili are important for biofilm formation and rough colony morphology. The third operon encodes the previously described Tap pili, which is homologous to the Pseudomonas aeruginosa type IV pili operon (Pil). The sequences of the latter two operons seem to be complete and uninterrupted. The fourth operon is similar to the E. coli Pap pili. We have created knock-out mutations in the Flp and Tap pili and have shown that they are not required for adhesion to fish cells in culture or for the formation of biofilms, however, they are important for fish pathogenesis. |
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