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ANNUAL CONFERENCE :: Abstract Library
Abstract Library
2003 Conference Abstract
| Type of Submission |
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Submission Type: |
Poster Presentation |
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Subject Category: |
Infection and Immunity |
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| Session Information |
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Presentation Date: |
May 27, 2003 |
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Abstract ID: |
D5 |
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Session: |
Poster 2 |
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Time: |
15:00 |
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| Presenting Author |
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| Other Authors |
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D.A. AL-NUMANI, Université de Montréal
S.L. LACOUTURE, Université de Montréal
M.S. SEGURA, Université Laval
M.G. GOTTSCHALK, Université de Montréal
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| Title |
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Induction of inflammatory cytokines and chemokines by Streptococcus suis in a porcine whole-blood model of infection. |
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| Abstract Text |
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Streptococcus suis serotype 2 is an important swine pathogen worldwide, and the cause of important economic losses. It is also a zoonotic agent associated to occupational diseases, causing meningitis and fulminate sepsis in humans. Inflammation is a hallmark of S. suis infection, and in this regard, it has been suggested that once bacteria enter into the CNS, the induction of an acute inflammatory exudates increases the volume of the cerebrospinal fluid, leading to an increased intracranial pressure, which is responsible for the clinical signs of meningitis. The objective of this work was to study the ability of S. suis type 2 to trigger the release of chemokines and inflammatory cytokines in a porcine whole blood model. Tumour Necrosis Factor alpha (TNFa), Interleukin-1 beta (Il-1ß ), Il-6, Il-8 and Monocyte Chemotactic Protein one (MCP1) induction was evaluated as the mRNA expression (RT-PCR) as well as the corresponding protein release (ELISA). All cytokines and chemiokines tested were induced by stimulation with S. suis. TNFa, Il-1ß and Il-8 mRNA expression quickly reached a plateau at 1h post-stimulation, while expression of Il-6 and MCP1 was slightly delayed. In general, protein levels correlated with mRNA expression. Levels of cytokines were higher with live than with heat-killed bacteria, and both were dose-dependant. Purified cell wall induced the release of all cytokines, while purified capsular polysaccharides and the hemolysin (suilysin) induced Il-1ß and chemokines, but not TNFa and IL-6. These results confirm that cell wall components are the major stimulants required for cytokines expression by S. suis activated cells. |
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