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ANNUAL CONFERENCE :: Abstract Library
Abstract Library
2003 Conference Abstract
| Type of Submission |
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Submission Type: |
Poster Presentation |
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Subject Category: |
Applied Microbiology |
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| Session Information |
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Presentation Date: |
May 26, 2003 |
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Abstract ID: |
A10 |
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Session: |
Poster 1 |
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Time: |
14:00 |
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| Presenting Author |
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J.A. GOW, Memorial University of Newfoundland
jgow@mun.ca |
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| Other Authors |
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J.E. TURPIN, Memorial University of Newfoundland
P.E. DABINETT, Memorial University of Newfoundland
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| Title |
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Antibiotic resistance in bacteria isolated from a Newfoundland and Labrador scallop hatchery |
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| Abstract Text |
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Vibriosis is an important disease affecting scallops in aquaculture hatcheries. Control, using antibiotics, is common and is most effective by treating the fertilized eggs and juvenal and metamorphosing animals. Treatment using a penicillin/streptomycin mixture or neomycin is common. However, vibrios are ubiquitous in hatcheries and information about strains that colonize a particular hatchery can provide the basis for the most effective treatment. For this study, 45 samples were obtained from several sites in the hatchery and bacterial isolates were selected following growth on TCBS agar. These were tested for their sensitivities to approximately 30 antibiotics belonging to eight antibiotic groups. The most effective antibiotics were polymyxin B (none resistant) and chloramphenicol (2% resistant). Of the aminoglycosides, resistance was least to gentamicin (5%) and, of the cephalosporins, resistance was least to cephalothin (10%). Of the tetracyclines, 7 to 9% of the strains were resistant to chlortetracycline, oxytetracycline and tetracycline. Resistance to sulfadiazine was 11%. Resistance to streptomycin, penicillin and neomycin was 89, 39, and 16%, respectively. Based on economic and other considerations, the tetracyclines were considered a good choice for treatment. Systematic studies and numerical analysis showed that the bacteria were predominately Vibrio species and approximately 70% of the strains were sensitive to 150 µg of the vibriostatic agent O/129. No relationship was shown between clusters of bacteria and their source in the hatchery. |
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