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ANNUAL CONFERENCE :: Abstract Library
Abstract Library
2003 Conference Abstract
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Submission Type: |
Poster Presentation |
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Subject Category: |
Applied Microbiology |
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| Session Information |
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Presentation Date: |
May 26, 2003 |
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Abstract ID: |
A8 |
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Session: |
Poster 1 |
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Time: |
14:00 |
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| Presenting Author |
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| Other Authors |
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J.C. CÔTÉ, Agriculture and Agri-Food Canada
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| Title |
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Distribution of transposable genetic elements IS231-like sequences among Bacillus thuringiensis serovars and strains |
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| Abstract Text |
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Bacillus thuringiensis is a gram-positive bacterium which upon sporulation produces a parasporal crystalline inclusion. Some of these inclusions have been shown to exhibit specific insecticidal activity against lepidopteran, dipteran, or coleopteran pests.
Insertion sequences (IS) are the simplest transposable elements, and usually contain only the genes responsible for their transposition. Some IS (IS231, IS232, IS233, IS240, ISBT1 and ISBT2) and two class II transposons (Tn4430 and Tn5401) have been isolated from a limited number of Bacillus thuringiensis strains. IS231 are found closely associated with cry genes and are believed to play a role in their multiple locations and genetic mobility, and in the generation of novel specificity.
We set to analyze the distribution of IS231-like sequences among Bacillus thuringiensis serovars. A total of 95 bacterial strains were tested in the present study. These include the 82 known B. thuringiensis serovars type strains, five more B. thuringiensis intra-serovar strains, kurstaki HD-1, subtoxicus, dendrolimus, tenebrionis and sandiego, a non-motile hence non-serotypeable strain, B. thuringiensis var wuhanensis, six B. cereus strains, and the B. mycoides type strain. A 723-bp HaeII conserved fragment from IS231M has been used as a probe against EcoRI-digested B. thuringiensis total DNA to yield serovar-specific hybridization profiles. The approach was useful at revealing the extent of distribution of IS231-like sequences between and within strains. Of the 88 B. thuringiensis strains tested, 70 showed hybridization banding patterns that comprised between one and 20 distinct bands. These 70 B. thuringiensis strains were grouped based on banding patterns similarities. Interestingly, intra-serovar strains did not necessarily cluster together.
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