| Type of Submission |
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Submission Type: |
Poster Presentation |
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Subject Category: |
Applied Microbiology |
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| Session Information |
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Presentation Date: |
May 26, 2003 |
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Abstract ID: |
A5 |
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Session: |
Poster 1 |
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Time: |
14:00 |
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| Presenting Author |
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L. LAGACÉ, Université de Montréal, Faculté de Médecine Vétérinaire. Centre de Recherche et de Développement des Aliments, Agriculture et Agro-Alimentaire Canada.
lagaceluc@agr.gc.ca |
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| Other Authors |
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M. PITRE, Centre de Recherche et de Développpement des Aliments, Agriculture et Agro-alimentaire Canada
D. ROY, Centre de Recherche et de Développement des Aliments, Agriculture et Agro-alimentaire Canada
M. JACQUES, Faculté de Médecine Vétérianire, Université de Montréal
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| Title |
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Bacterial community identification of maple sap as determined by ARDRA and ribosomal DNA sequencing. |
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| Abstract Text |
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Maple sap is known to be contaminated by bacteria that will eventually affect the productivity and the quality of maple syrup and other maple products. The maple tree taphole is the first point of entry into the sap for bacteria. Better knowledge of taphole bacteria diversity and identity would undoubtedly improve strategies to control taphole contamination. Therefore, 160 bacterial isolates were recovered from maple sap samples taken at the taphole over 5 sampling periods during the 2001 season. Microbial counts of the samples demonstrate that contamination increased during the season and that bacteria were dominant as compared to yeasts and moulds. The bacterial isolates were further analysed by Amplified Ribosomal DNA Restriction Analysis (ARDRA). Dendrogram based on the ARDRA restriction profiles indicates that isolates were grouped into 29 different clusters. Cloning and partial sequencing of 16S rDNA from representative isolates of each cluster reveals that Burkholderia spp. and Pseudomonas spp. for Gram negative and Staphylococcus spp. for Gram positive are among the prevailing genera found in maple sap. Shannon index calculation also shows that diversity of maple sap bacterial isolates is higher at mid-season compared to early and late season. These results are providing insight into the microbial ecology of maple sap and will be useful for developing contamination control approaches. |
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