| Type of Submission |
 |
Submission Type: |
Poster Presentation |
|
Subject Category: |
Veterinary Microbiology |
|
| Session Information |
|
Presentation Date: |
May 26, 2003 |
|
Abstract ID: |
G4 |
|
Session: |
Poster 1 |
|
Time: |
14:00 |
|
| Presenting Author |
|
| Other Authors |
|
KR MITTAL, Faculte medecine veterinaire Universite de Montreal
S BOURDON, Faculte medecine veterinaire Universite de Montreal
M GOTTSCHALK, Faculte medecine veterinaire Universite de Montreal
|
|
| Title |
|
Production of monoclonal antibodies against Haemophilus parasuis |
|
| Abstract Text |
|
Monoclonal antibodies (MAbs) against H. parasuis were obtained by fusion of SP2/0-Ag14 murine myeloma cells and spleen cells of Balb/c mice immunized with whole bacterial cell (WC) suspension of H. parasuis strain SW140 of serotype 2. Thirty two hybridomas were selected by Enzyme-Linked ImmunoSorbent Assay (ELISA) using sonicated antigen. Two MAbs showing strong reactivity in ELISA were further characterized using SDS-PAGE and Western-Blot assay. Treatment of WC with proteinase K and sodium periodate indicated that MAb 1 (4D5) and MAb 2 (4G9) identified epitopes of proteinic and polysaccharidic nature respectively. The MAb 4D5 reacted with common out membrane protein epitopes of all the reference and some field strains of H. parasuis tested. None of the other Gram negative bacteria reacted with this MAb. These results indicated that this protein carried a species-specific epitopes and MAb 4D5 may be useful for identification of H. parasuis. Immunogold labelling studie showed that the antibody-binding epitopes were exposed on the outer-surface of the H. parasuis cell wall. This is the first report of the MAb identifying and characterizing common antigens of all the reference strains of H. parasuis. The second MAb (4G9) recognised polysaccharidic epitopes shared only by some reference strains. |
|
